A RAPID AND SIMPLE METHOD FOR THE PURIFICATION OF TRANSGLUTAMINASE FROM STREPTOVERTICILLIUM MOBARAENSE

被引：71

作者：

GERBER, U ^{[1
]}

JUCKNISCHKE, U ^{[1
]}

PUTZIEN, S ^{[1
]}

FUCHSBAUER, HL ^{[1
]}

机构：

[1] FACHHSCH DARMSTADT, FACHBEREICH CHEM TECHNOL, D-64289 DARMSTADT, GERMANY

来源：

BIOCHEMICAL JOURNAL | 1994年 / 299卷

关键词：

D O I：

10.1042/bj2990825

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transglutaminase from Streptoverticillium mobaraense was partially purified by ion-exchange chromatography on a weak acid material and hydrophobic chromatography. The separation with a strong acid ion-exchanger produces homogeneous transglutaminase, in a single step and with high yields, directly from the centrifuged and filtered culture fluid of the micro-organism. The procedure reproduced several times could be also carried out on a larger scale with the optimized parameters of the laboratory isolations. The purified enzyme demonstrated good storage stability.

引用

页码：825 / 829

页数：5

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