Effect of temperature on the growth, total lipid content and fatty acid composition of recently isolated tropical microalgae Isochrysis sp, Nitzschia closterium, Nitzschia paleacea, and commercial species Isochrysis sp (clone T ISO)

被引:152
作者
Renaud, SM
Zhou, HC
Parry, DL
Thinh, LV
Woo, KC
机构
[1] SHANGHAI FISHERIES UNIV, DEPT AQUACULTURE, SHANGHAI, PEOPLES R CHINA
[2] NO TERR UNIV, FAC SCI, SCH BIOL SCI, DARWIN, NT, AUSTRALIA
关键词
microalgae; temperature; growth; total lipids; fatty acids; tropical mariculture;
D O I
10.1007/BF00003948
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The effect of temperature from 10 degrees C to 35 degrees C on the growth, total lipid content, and fatty acid composition of three species of tropical marine microalgae, Isochrysis sp., Nitzschia closterium, N. paleacea (formerly frustulum), and the Tahitian Isochrysis sp. (T.ISO), was investigated. Cultures of N. closterium, Isochrysis sp. and T.ISO grew very slowly at 35 degrees C, while N. closterium did not grow at temperatures higher than 30 degrees C or lower than 20 degrees C. N. paleacea was low-temperature tolerant, with cells growing slowly at 10 degrees C. N. paleacea produced the highest percentage of lipids at 10 degrees C, while the other species produced maximum amounts of lipid at 20 degrees C. None of the species maintained high levels of polyunsaturated fatty acids (PUFAs) at high growth temperature and there was a significant inverse relationship between the percentage of PUFAs and temperature for N. paleacea. A curved relationship was found between temperature and percentage of PUFA for N. closterium and tropical Isochrysis sp., with the maximum production of PUFA at 25 degrees C and 20 degrees C, respectively. The two Nitzschia species produced higher levels of the essential fatty acid eicosapentaenoic acid [20:5(n-3)] at lower growth temperatures, but the two Isochrysis species had little change in percentage of 20:5(n-3) with temperature. Only T.ISO had the highest percentage of 22:6(n-3) at lowest growth temperature (11.4% total fatty acids at 10 degrees C).
引用
收藏
页码:595 / 602
页数:8
相关论文
共 18 条
[1]  
Aaronson S., Effect of incubation temperature on the macromolecular and lipid content of the phytoflagellate Ochromonas danica, Journal of Phycology, 9, pp. 111-113, (1973)
[2]  
Ackman R.G., Tocher C.S., McLachlan J., Marine phytoplankton fatty acids, Journal of the Fisheries Research Board of Canada, 25, pp. 1603-1620, (1968)
[3]  
Bligh E.G., Dyer W.J., A rapid method of total lipid extraction and purification, Can. J. Biochem. Physiol., 37, pp. 911-917, (1959)
[4]  
Guillard R.R.L., Methods for microflagellates and nanoplankton, Handbook of Phycological Methods, pp. 80-81, (1973)
[5]  
Guillard R.R.L., Ryther J.H., Studies on marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detinula confervacea (Cleve) Gran, Can. J. Microbiol., 18, pp. 229-239, (1962)
[6]  
Harwood J.L., Fatty acid metabolism, Annual Review of Plant Physiology and Plant Molecular Biology, 39, pp. 101-138, (1988)
[7]  
James C.M., Al-Hinty S., Salman A.E., Growth and ω3 fatty acid and amino acid composition of microalgae under different temperature regimes, Aquaculture, 77, pp. 337-351, (1989)
[8]  
Materassi R., Paoletti C., Balloni W., Florenzano G., Some considerations on the production of lipid substances by microalgae and cyanobacteria, Algal Biomass, pp. 619-626, (1980)
[9]  
Mortensen S.H., Borsheim K.Y., Rainuzzo J.K., Knutsen G., Fatty acid and elemental composition of the marine diatom Chaetoceros gracilis Schutt. Effects of silicate deprivation, temperature and light intensity, J. exp. mar. Biol. Ecol., 122, pp. 173-185, (1988)
[10]  
Opute F.I., Studies on fat accumulation in Nitzschia palea Kutz, Ann. Bot., 38, pp. 889-902, (1974)