EXPRESSION OF PSEUDOMONAS PHOSPHOTRIESTERASE ACTIVITY IN THE FALL ARMYWORM CONFERS RESISTANCE TO INSECTICIDES

被引：16

作者：

DUMAS, DP

WILD, JR

RAUSHEL, FM

机构：

[1] TEXAS A&M UNIV SYST,DEPT CHEM,COLLEGE STN,TX 77843

[2] TEXAS A&M UNIV SYST,BIOCHEM & BIOPHYS,COLLEGE STN,TX 77843

来源：

EXPERIENTIA | 1990年 / 46卷 / 07期

关键词：

fall armyworm; paraoxon; pesticide resistance; Phosphotriesterase;

D O I：

10.1007/BF01939948

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The gene encoding for the phosphotriesterase (opd) from Pseudomonas diminuta has been subcloned into a baculovirus expression system. Functional enzyme is produced when the recombinant baculovirus is used to infect either cultured Spodoptera frugiperda sf9 cells or the larval stage of the fall armyworm. The LD50 for paraoxon toxicity was found to increase 280-fold in the larvae after infection with the recombinant baculovirus and expression of the functional phosphotriesterase. © 1990 Birkhäuser Verlag Basel.

引用

页码：729 / 731

页数：3

共 14 条

[1] AN ALTERNATIVE SYSTEM FOR CLASSIFICATION OF MATHEMATICAL MODELS FOR QUANTAL RESPONSES TO MIXTURES OF DRUGS IN BIOLOGICAL ASSAY [J].