A SPECIFIC HARMALINE-EVOKED INCREASE IN CEREBELLAR 5'-NUCLEOTIDASE ACTIVITY

被引：7

作者：

BALABAN, CD ^{[1
]}

WURPEL, JND ^{[1
]}

SEVERS, WB ^{[1
]}

机构：

[1] PENN STATE UNIV, MILTON S HERSHEY MED CTR, DEPT PHARMACOL, HERSHEY, PA 17033 USA

来源：

NEUROSCIENCE LETTERS | 1984年 / 50卷 / 1-3期

关键词：

D O I：

10.1016/0304-3940(84)90471-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Harmaline-induced changes in 5''-nucleotidase (5''-ND) activity were examined in cerebellar fractions from rats with an intact inferior olive (IO) or prior destruction of the IO by 3-acetylpyridine (3-AP) intoxication. Harmaline markedly increased 5''-ND activity in the crude homogenate (P < 0.05) and P2 fraction (P < 0.001) of cerebella from rats with an intact IO. This increased was absent in the P1, P3 and S3 fractions and it was abolished by 3-AP olivectomy. It was also absent in basal ganglia P2 fractions. Since harmaline produces rhythmic complex spike discharges of Purkinje cells by activating IO neurons climbing fiber activation per se increases 5''-ND activity in the P2 fraction. A climbing fiber-induced local increase in 5''-ND activity at parallel fiber-Purkinje cell synapses may result in a local increases in adenosine concentration. This may account for climbing fiber-evoked suppression of simple spike activity.

引用

页码：111 / 116

页数：6

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