GROUP SEPARATION OF FREE AND CONJUGATED BILE-ACIDS BY PRE-PACKED ANION-EXCHANGE CARTRIDGES

被引：20

作者：

SCALIA, S

机构：

[1] Dipartimento di Scienze Farmaceutiche, Università di Ferrara, 44100 Ferrara

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1990年 / 8卷 / 03期

关键词：

Anion-exchange cartridges; determination in human bile and gastric juice; free and conjugated bile acids; group separation; high-performance liquid chromatography;

D O I：

10.1016/0731-7085(90)80032-K

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid and simple procedure for the group separation of the major free and conjugated bile acids of man is described. After initial extraction with Bond Elut C18 cartridges, the analytes are fractionated into the unconjugated, glycine- and taurine-conjugated forms using disposable Bond Elut SAX columns and methanol-acetate buffer eluents. The method is found to be accurate and reproducible and to afford complete resolution between fractions. Free and conjugated bile acids present in human bile and gastric juice are assayed by high-performance liquid chromatography, after extraction and group separation according to the described procedure. © 1990.

引用

页码：235 / 241

页数：7

共 23 条

[1]

ALME B, 1977, J LIPID RES, V18, P339

[2] QUANTITATION OF FREE AND CONJUGATED BILE-ACIDS IN HUMAN FECES USING A HIGH-PRESSURE LIQUID-CHROMATOGRAPHY COUNTERION METHOD [J].