EVIDENCE THAT FIBROBLAST GROWTH FACTOR-5 IS A MAJOR MUSCLE-DERIVED SURVIVAL FACTOR FOR CULTURED SPINAL MOTONEURONS

被引：128

作者：

HUGHES, RA ^{[1
]}

SENDTNER, M ^{[1
]}

GOLDFARB, M ^{[1
]}

LINDHOLM, D ^{[1
]}

THOENEN, H ^{[1
]}

机构：

[1] COLUMBIA UNIV COLL PHYS & SURG,DEPT BIOCHEM & MOLEC BIOPHYS,NEW YORK,NY 10032

来源：

NEURON | 1993年 / 10卷 / 03期

关键词：

D O I：

10.1016/0896-6273(93)90327-N

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We examined the potential role of fibroblast growth factor 5 (FGF-5) as a target-derived trophic factor for spinal motoneurons. Northern analysis of total RNA from rat skeletal muscle revealed an FGF-5 mRNA transcript both during the period of embryonic motoneuron death and in the adult. Recombinant human FGF-5 supported the survival of highly enriched cultures of embryonic chick motoneurons. Significant proportions of the motoneuron survival activity of rat skeletal muscle extracts could be immunoprecipitated using an antiserum to FGF-5. The immunoprecipitable activity was present in soluble and matrix-bound forms in embryonic muscle, but bound exclusively to the extracellular matrix in adult muscle. These results, along with the secretory nature of FGF-5, suggest that FGF-5 may act as a target-derived trophic factor for motoneurons.

引用

页码：369 / 377

页数：9

共 68 条

[1] NUCLEOTIDE-SEQUENCE OF A BOVINE CLONE ENCODING THE ANGIOGENIC PROTEIN, BASIC FIBROBLAST GROWTH-FACTOR [J].

ABRAHAM, JA ;

MERGIA, A ;

WHANG, JL ;

TUMOLO, A ;

FRIEDMAN, J ;

HJERRILD, KA ;

GOSPODAROWICZ, D ;

FIDDES, JC .

SCIENCE, 1986, 233 (4763) :545-548

[2] ACIDIC AND BASIC FIBROBLAST GROWTH-FACTOR MESSENGER-RNAS ARE EXPRESSED BY SKELETAL-MUSCLE SATELLITE CELLS [J].

ALTERIO, J ;

COURTOIS, Y ;

ROBELIN, J ;

BECHET, D ;

MARTELLY, I .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 166 (03) :1205-1212

[3]

ARAKAWA Y, 1990, J NEUROSCI, V10, P3507

[4] TROPHIC FACTORS AND NEURONAL SURVIVAL [J].

BARDE, YA .

NEURON, 1989, 2 (06) :1525-1534

[5] BIOSYNTHESIS OF HUMAN FIBROBLAST GROWTH FACTOR-5 [J].

BATES, B ;

HARDIN, J ;

ZHAN, X ;

DRICKAMER, K ;

GOLDFARB, M .

MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (04) :1840-1845

[6] IDENTIFICATION OF EMBRYONIC MOTO-NEURONS INVITRO - THEIR SURVIVAL IS DEPENDENT ON SKELETAL-MUSCLE [J].

BENNETT, MR ;

LAI, K ;

NURCOMBE, V .

BRAIN RESEARCH, 1980, 190 (02) :537-542

[7]

BLOCHGALLEGO E, 1991, DEVELOPMENT, V111, P221

[8]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[9] MOTONEURONS PURIFIED BY CELL SORTING RESPOND TO 2 DISTINCT ACTIVITIES IN MYOTUBE-CONDITIONED MEDIUM [J].

CALOF, AL ;

REICHARDT, LF .

DEVELOPMENTAL BIOLOGY, 1984, 106 (01) :194-210

[10]

CASTREN E, 1992, IN PRESS P NATL ACAD

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