PURIFICATION, CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF THE 3-PHOSPHOGLYCERATE KINASE FROM BACILLUS-STEAROTHERMOPHILUS

被引：4

作者：

DAVIES, GJ ^{[1
]}

GAMBLIN, SJ ^{[1
]}

LITTLECHILD, JA ^{[1
]}

WATSON, HC ^{[1
]}

机构：

[1] UNIV BRISTOL,SCH MED SCI,DEPT BIOCHEM,BRISTOL BS8 1TD,AVON,ENGLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 227卷 / 04期

关键词：

PHOSPHOGLYCERATE KINASE; BACILLUS-STEAROTHERMOPHILUS; CRYSTALS; THERMAL STABILITY; X-RAY DIFFRACTION ANALYSIS;

D O I：

10.1016/0022-2836(92)90538-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

As part of a programme investigating the molecular basis of thermal stability in proteins we have isolated and characterized the thermally stable 3-phosphoglycerate kinase (PGK) from Bacillus stearothermophilus NCA 1503. The B. stearothermophilus PGK has been crystallized in a form suitable for X-ray diffraction analysis. Crystals which diffract to greater than 1·8 Å resolution have been grown in the presence of the nucleotide substrate, MgATP, using polyethylene glycol (PEG 600) as a precipitant. The best crystals have been obtained using "seeding" techniques and are monoclinic, space group P21, with cell dimensions a = 40·5 A ̊, b = 74·0 A ̊, c = 68·5 A ̊ and β = 99·8 °. © 1992.

引用

页码：1263 / 1264

页数：2

共 9 条

[1] SEQUENCE, STRUCTURE AND ACTIVITY OF PHOSPHOGLYCERATE KINASE - POSSIBLE HINGE-BENDING ENZYME
BANKS, RD
BLAKE, CCF
EVANS, PR
HASER, R
RICE, DW
HARDY, GW
MERRETT, M
PHILLIPS, AW
[J]. NATURE, 1979, 279 (5716) : 773 - 778
[2] NUCLEOTIDE-SEQUENCE OF THE PHOSPHOGLYCERATE KINASE GENE FROM THE EXTREME THERMOPHILE THERMUS-THERMOPHILUS - COMPARISON OF THE DEDUCED AMINO-ACID SEQUENCE WITH THAT OF THE MESOPHILIC YEAST PHOSPHOGLYCERATE KINASE
BOWEN, D
LITTLECHILD, JA
FOTHERGILL, JE
WATSON, HC
HALL, L
[J]. BIOCHEMICAL JOURNAL, 1988, 254 (02) : 509 - 517
[3] SEQUENCE AND EXPRESSION OF THE GENE ENCODING 3-PHOSPHOGLYCERATE KINASE FROM BACILLUS-STEAROTHERMOPHILUS
DAVIES, GJ
LITTLECHILD, JA
WATSON, HC
HALL, L
[J]. GENE, 1991, 109 (01) : 39 - 45
[4] THE PURIFICATION AND CHARACTERIZATION OF GLUCOKINASE FROM THE THERMOPHILE BACILLUS-STEAROTHERMOPHILUS
GOWARD, CR
HARTWELL, R
ATKINSON, T
SCAWEN, MD
[J]. BIOCHEMICAL JOURNAL, 1986, 237 (02) : 415 - 420
[5] MATURATION OF HEAD OF BACTERIOPHAGE-T4 .1. DNA PACKAGING EVENTS
LAEMMLI, UK
FAVRE, M
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1973, 80 (04) : 575 - 599
[6] PHOSPHOGLYCERATE KINASE FROM THE EXTREME THERMOPHILE THERMUS-THERMOPHILUS - CRYSTALLIZATION AND PRELIMINARY-X-RAY DATA
LITTLECHILD, JA
DAVIES, GJ
GAMBLIN, SJ
WATSON, HC
[J]. FEBS LETTERS, 1987, 225 (1-2) : 123 - 126
[7] SOLVENT CONTENT OF PROTEIN CRYSTALS
MATTHEWS, BW
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1968, 33 (02) : 491 - +
[8] SEQUENCE AND STRUCTURE OF YEAST PHOSPHOGLYCERATE KINASE
WATSON, HC
WALKER, NPC
SHAW, PJ
BRYANT, TN
WENDELL, PL
FOTHERGILL, LA
PERKINS, RE
CONROY, SC
DOBSON, MJ
TUITE, MF
KINGSMAN, AJ
KINGSMAN, SM
[J]. EMBO JOURNAL, 1982, 1 (12) : 1635 - 1640
[9] ISOENZYMES OF PHOSPHOGLYCERATE KINASE - EVOLUTIONARY CONSERVATION OF THE STRUCTURE OF THIS GLYCOLYTIC ENZYME
WATSON, HC
LITTLECHILD, JA
[J]. BIOCHEMICAL SOCIETY TRANSACTIONS, 1990, 18 (02) : 187 - 190

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