ENZYMATIC METHOD FOR THE SPECTROPHOTOMETRIC DETERMINATION OF CHOLINE IN LIQUOR

被引：7

作者：

HAMANO, T

MITSUHASHI, Y

AOKI, N

YAMAMOTO, S

SHIBATA, M

ITO, Y

OJI, Y

LIAN, WF

机构：

[1] NATL INST HYG SCI, OSAKA BRANCH, CHUO KU, OSAKA 540, JAPAN

[2] KOBE UNIV, FAC AGR, KOBE 657, JAPAN

[3] JABATAN KIMIA MALAYSIA, JALAN SULTAN, MALAYSIA

来源：

ANALYST | 1992年 / 117卷 / 06期

关键词：

CHOLINE DETERMINATION; CHOLINE OXIDASE; CATALASE;

D O I：

10.1039/an9921701033

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive spectrophotometric method for the determination of choline in liquor is described. The method involves the conversion of choline into formaldehyde by sequential enzymic reactions (choline oxidase and catalase), followed by the formation of a chromophore with 4-aminopent-3-en-2-one. The calibration graph was linear in the range 0.4-15-mu-g cm-3 of choline. The relative standard deviation at 5-mu-g cm-3 of choline was 1.3%. There was no interference from most of the common ingredients of liquor. More than 95% of choline added at two levels was recovered from real samples. The method is simple, and the detection limit was 2-mu-g g-1 when 5 g of sample were assayed.

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页码：1033 / 1035

页数：3

相关论文

共 9 条

[1] FLUORAL-P, A MEMBER OF A SELECTIVE FAMILY OF REAGENTS FOR ALDEHYDES [J].

COMPTON, BJ ;

PURDY, WC .

ANALYTICA CHIMICA ACTA, 1980, 119 (02) :349-357

[2] REVERSED-PHASE, ION-PAIR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF AMINOETHANOL, CHOLINE AND RELATED-COMPOUNDS, AND A DIRECT, QUANTITATIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR CHOLINE IN PLANTS [J].

GORHAM, J .

JOURNAL OF CHROMATOGRAPHY, 1986, 362 (02) :243-253

[3] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE SEPARATION AND ESTIMATION OF CHOLINE, GLYCINEBETAINE ALDEHYDE AND RELATED-COMPOUNDS [J].

GORHAM, J ;

MCDONNELL, E .

JOURNAL OF CHROMATOGRAPHY, 1985, 350 (01) :245-254

[4] DETERMINATION OF GLYCEROL IN FOODS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION [J].

HAMANO, T ;

MITSUHASHI, Y ;

AOKI, N ;

YAMAMOTO, S ;

TSUJI, S ;

ITO, Y ;

OJI, Y .

JOURNAL OF CHROMATOGRAPHY, 1991, 541 (1-2) :265-272

[5] DETERMINATION OF CHOLINE AND ACETYLCHOLINE LEVELS IN RAT-BRAIN REGIONS BY LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION [J].

IKARASHI, Y ;

SASAHARA, T ;

MARUYAMA, Y .

JOURNAL OF CHROMATOGRAPHY, 1985, 322 (01) :191-199

[6] SIMPLE METHOD FOR THE SIMULTANEOUS DETERMINATION OF ACETYLCHOLINE, CHOLINE, NORADRENALINE, DOPAMINE AND SEROTONIN IN BRAIN-TISSUE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION [J].

KANEDA, N ;

ASANO, M ;

NAGATSU, T .

JOURNAL OF CHROMATOGRAPHY, 1986, 360 (01) :211-218

[7] IMPROVEMENTS IN THE GAS-CHROMATOGRAPHIC ANALYSIS OF ACETYLCHOLINE AND CHOLINE [J].

KOSH, JW ;

SMITH, MB ;

SOWELL, JW ;

FREEMAN, JJ .

JOURNAL OF CHROMATOGRAPHY, 1979, 163 (02) :206-211

[8]

MACINTOSH FC, 1950, METH MED RES, V3, P78

[9]

TAKAYAMA M, 1977, CLIN CHIM ACTA, V79, P93, DOI 10.1016/0009-8981(77)90465-X