A MULTIPLEX PCR METHOD FOR THE IDENTIFICATION OF LISTERIA SPP AND LISTERIA-MONOCYTOGENES IN DAIRY SAMPLES

被引：34

作者：

HERMAN, LMF

DERIDDER, HFM

VLAEMYNCK, GMM

机构：

[1] Government Dairy Research Station, Agricultural Research Center, Ghent

来源：

JOURNAL OF FOOD PROTECTION | 1995年 / 58卷 / 08期

关键词：

PCR; LISTERIA SPP; LISTERIA MONOCYTOGENES;

D O I：

10.4315/0362-028X-58.8.867

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A multiplex polymerase chain reaction (PCR) method for the identification of Listeria spp. and Listeria monocytogenes has been developed. For the identification of Listeria spp. two specific primers were derived from the 16S rRNA sequence. For the identification of Listeria monocytogenes specific primers were derived from the gene sequence of the invasion-associated protein. The two amplified DNA fragments, respectively 1003 bp and 593 bp, were separated by agarose electrophoresis. The results obtained by the multiplex PCR method were in agreement with those obtained by the classical bacteriological method for the 64 dairy samples with presumptive positives.

引用

收藏

页码：867 / 872

页数：6

相关论文

共 21 条

[1] PHYLOGENETIC HETEROGENEITY OF THE GENUS BACILLUS REVEALED BY COMPARATIVE-ANALYSIS OF SMALL-SUBUNIT-RIBOSOMAL RNA SEQUENCES [J].

ASH, C ;

FARROW, JAE ;

WALLBANKS, S ;

COLLINS, MD .

LETTERS IN APPLIED MICROBIOLOGY, 1991, 13 (04) :202-206

[2] LISTERIA-MONOCYTOGENES MENINGITIS ASSOCIATED WITH EATING SOFT CHEESE [J].

BANNISTER, BA .

JOURNAL OF INFECTION, 1987, 15 (02) :165-168

[3] DETECTION OF LISTERIA-MONOCYTOGENES BY USING THE POLYMERASE CHAIN-REACTION [J].

BESSESEN, MT ;

LUO, Q ;

ROTBART, HA ;

BLASER, MJ ;

ELLISON, RT .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (09) :2930-2932

[4] DETECTION OF LISTERIA SPECIES AND LISTERIA-MONOCYTOGENES USING POLYMERASE CHAIN-REACTION [J].

BORDER, PM ;

HOWARD, JJ ;

PLASTOW, GS ;

SIGGENS, KW .

LETTERS IN APPLIED MICROBIOLOGY, 1990, 11 (03) :158-162

[5]

BOSSUYT R, 1977, REV AGR-BRUSSELS, V30, P919

[6] PHYLOGENETIC ANALYSIS OF THE GENUS LISTERIA BASED ON REVERSE-TRANSCRIPTASE SEQUENCING OF 16S RIBOSOMAL-RNA [J].

COLLINS, MD ;

WALLBANKS, S ;

LANE, DJ ;

SHAH, J ;

NIETUPSKI, R ;

SMIDA, J ;

DORSCH, M ;

STACKEBRANDT, E .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1991, 41 (02) :240-246

[7] SYNTHETIC OLIGODEOXYRIBONUCLEOTIDE PROBES FOR DETECTION OF LISTERIA-MONOCYTOGENES [J].

DATTA, AR ;

WENTZ, BA ;

SHOOK, D ;

TRUCKSESS, MW .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (12) :2933-2937

[8] SELECTIVE-ENRICHMENT PROCEDURE FOR ISOLATION OF LISTERIA-MONOCYTOGENES FROM FECAL AND BIOLOGIC SPECIMENS [J].

DOYLE, MP ;

SCHOENI, JL .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1986, 51 (05) :1127-1129

[9] INTRODUCTION OF PAM-BETA-1 INTO LISTERIA-MONOCYTOGENES BY CONJUGATION AND HOMOLOGY BETWEEN NATIVE L-MONOCYTOGENES PLASMIDS [J].

FLAMM, RK ;

HINRICHS, DJ ;

THOMASHOW, MF .

INFECTION AND IMMUNITY, 1984, 44 (01) :157-161

[10] PASTEURIZED MILK AS A VEHICLE OF INFECTION IN AN OUTBREAK OF LISTERIOSIS [J].

FLEMING, DW ;

COCHI, SL ;

MACDONALD, KL ;

BRONDUM, J ;

HAYES, PS ;

PLIKAYTIS, BD ;

HOLMES, MB ;

AUDURIER, A ;

BROOME, CV ;

REINGOLD, AL .

NEW ENGLAND JOURNAL OF MEDICINE, 1985, 312 (07) :404-407