THE ISOMALTULOSE SYNTHESIZING ENZYME OF SERRATIA-PLYMUTHICA

被引：32

作者：

MCALLISTER, M ^{[1
]}

KELLY, CT ^{[1
]}

DOYLE, E ^{[1
]}

FOGARTY, WM ^{[1
]}

机构：

[1] NATL UNIV IRELAND UNIV COLL DUBLIN,DEPT IND MICROBIOL,DUBLIN 4,IRELAND

来源：

BIOTECHNOLOGY LETTERS | 1990年 / 12卷 / 09期

关键词：

D O I：

10.1007/BF01088191

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An intracellular enzyme was located in Serratiaplymuthica which produced isomaltulose from sucrose. The enzyme was purified giving a preparation with a specific activity of 1,285. It has pH and temperature optima of 6.0 and 30°C, respectively. The enzyme was stable retaining 100% activity after 2 weeks at 30°C. It had an isoelectric point at pH 9.0, a Mr of 79,500 and the Km for sucrose was 65.3mM. The enzyme converted 40% (w/v) sucrose to isomaltulose with an efficiency of 87%. © 1990 Kluwer Academic Publishers.

引用

页码：667 / 672

页数：6

共 18 条

[1] ESTIMATION OF MOLECULAR WEIGHTS OF PROTEINS BY SEPHADEX GEL-FILTRATION [J].

ANDREWS, P .

BIOCHEMICAL JOURNAL, 1964, 91 (02) :222-+

[2] AMYLASES, ALPHA AND BETA [J].

BERNFELD, P .

METHODS IN ENZYMOLOGY, 1955, 1 :149-158

[3] THE FORMATION OF ISOMALTULOSE BY IMMOBILIZED ERWINIA-RHAPONTICI [J].

CHEETHAM, PSJ ;

IMBER, CE ;

ISHERWOOD, J .

NATURE, 1982, 299 (5884) :628-631

[4]

CHEETHAM PSJ, 1984, BIOTECHNOL BIOENG, V28, P471

[5]

CHEETHAM PSJ, 1984, BIOCHEM J, V280, P213

[6]

HANSEN SA, 1975, J CHROMATOGR, V107, P324

[7]

Huggett A. S. C., 1955, BIOCHEM J, V66, P12

[8]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[9]

Nakajima Y., 1984, Proceedings of the Research Society of Japan Sugar Refineries Technologists [Seito Gijutsu Kenkyukai-shi], V33, P55

[10]

ROBERTS PG, 1980, SCAND J DENT RES, V24, P201

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