APPLICATION OF INVITRO POLLINATION, OVARY CULTURE, OVULE CULTURE AND EMBRYO RESCUE FOR OVERCOMING INCONGRUITY BARRIERS IN INTERSPECIFIC LILIUM CROSSES

The use of a complete and integrated in vitro pollination, fertilization and embryo rescue system in lily was examined. By combining pollination techniques to overcome pre-fertilization barriers with in vitro methods to overcome post-fertilization barriers, interspecific lily crosses could be made more efficiently. In vitro cut-style pollination and in vitro grafted style technique were developed and applied on various interspecific crosses using Lilium longiflorum, L. dauricum, L. henryi, and both Asiatic and Oriental hybrids as the parents. In addition, methods for ovary culture, ovary-slice culture and ovule culture were generated. Ovule swelling score in ovary culture was used to evaluate media effects on ovule development. Using the integrated in vitro pollination and fertilization protocol it was not only possible to raise the total number of hybrid plantlets in a single interspecific cross, but also the number of successful interspecific combinations.