SIMULTANEOUS PURIFICATION OF THE NEUROPROTEINS SYNAPSIN-I AND SYNAPTOPHYSIN

被引：10

作者：

LLONA, I ^{[1
]}

ANNAERT, WG ^{[1
]}

DEPOTTER, WP ^{[1
]}

机构：

[1] UNIV INSTELLING ANTWERP, DEPT MED, NEUROPHARMACOL LAB, UNIV PLEIN 1, B-2610 Antwerp, BELGIUM

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 596卷 / 01期

关键词：

D O I：

10.1016/0021-9673(92)80201-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A procedure for the simultaneous purification of synapsin I and synaptophysin from calf brain was developed. Demyelinated membranes were extracted with 2% Triton X-100 and 2 M KCl. The extracted proteins were separated by weak cation-exchange chromatography on carboxymethyl-Sepharose Fast Flow. Synaptophysin was finally purified by preparative sodium dodecyl sulphate-polyacrylamine gel electrophoresis and synapsin I by affinity chromatography using a calmodulin-Sepharose column. The recovery obtained was 40-mu-g/g in brain for synaptophysin and 25-mu-g/g in brain for synapsin I.

引用

页码：51 / 58

页数：8

共 36 条

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