REQUIREMENT FOR TRANSCRIPTION FACTOR IRF-1 IN NO SYNTHASE INDUCTION IN MACROPHAGES

被引：784

作者：

KAMIJO, R

HARADA, H

MATSUYAMA, T

BOSLAND, M

GERECITANO, J

SHAPIRO, D

LE, J

KOH, SI

KIMURA, T

GREEN, SJ

MAK, TW

TANIGUCHI, T

VILCEK, J

机构：

[1] NYU MED CTR,DEPT MICROBIOL,NEW YORK,NY 10016

[2] NYU MED CTR,KAPLAN CANC CTR,NEW YORK,NY 10016

[3] OSAKA UNIV,INST MOLEC & CELLULAR BIOL,OSAKA 565,JAPAN

[4] UNIV TORONTO,PRINCESS MARGARET HOSP,ONTARIO CANC INST,DEPT MED BIOPHYS,TORONTO M4X 1K9,ON,CANADA

[5] UNIV TORONTO,PRINCESS MARGARET HOSP,ONTARIO CANC INST,DEPT IMMUNOL,TORONTO M4X 1K9,ON,CANADA

[6] NYU MED CTR,INST ENVIRONM MED,TUXEDO PK,NY 10987

[7] NYU MED CTR,KAPLAN CANC CTR,TUXEDO PK,NY

[8] ENTREMED INC,ROCKVILLE,MD 20850

来源：

SCIENCE | 1994年 / 263卷 / 5153期

关键词：

D O I：

10.1126/science.7510419

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Production of nitric oxide (NO) by macrophages is important for the killing of intracellular infectious agents. Interferon (IFN)-gamma and lipopolysaccharide stimulate NO production by transcriptionally up-regulating the inducible NO synthase (iNOS). Macrophages from mice with a targeted disruption of the IFN regulatory factor-1 (IRF-1) gene (IRF-1(-/-) mice) produced little or no NO and synthesized barely detectable iNOS messenger RNA in response to stimulation. Two adjacent IRF-1 response elements were identified in the iNOS promoter. Infection with Mycobacterium bovis (BCG) was more severe in IRF-1(-/-) mice than in wild-type mice. Thus, IRF-1 is essential for iNOS activation in murine macrophages.

引用

页码：1612 / 1615

页数：4

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