GROWTH-CHARACTERISTICS OF GLYCYRRHIZA-GLABRA CELL-SUSPENSION CULTURES

被引：12

作者：

ARIASCASTRO, C

SCRAGG, AH

STAFFORD, A

RODRIGUEZMENDIOLA, M

机构：

[1] UNIV W ENGLAND, FAC APPL SCI, BRISTOL S10 2TN, ENGLAND

[2] UNIV SHEFFIELD, DEPT MOLEC BIOL & BIOTECHNOL, WESTERN BANK, SHEFFIELD S10 2TN, S YORKSHIRE, ENGLAND

[3] WESTERN BANK, PLANT SCI LTD, SHEFFIELD S10 2TN, ENGLAND

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1993年 / 34卷 / 01期

关键词：

CALLUS CULTURES; CULTURE CHARACTERISTICS; GLYCYRRHIZA-GLABRA; SUSPENSION CULTURES;

D O I：

10.1007/BF00048466

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Callus and cell suspension cultures of licorice (Glycyrrhiza glabra) have been established in Gamborg's B5 medium supplemented with 2% sucrose, 1.0 mg l-1 2,4-dichlorophenoxyacetic acid and 0.1 mg l-1 kinetin. Biomass accumulation was affected by the density and age of inoculum, vessel size and sucrose level. 8% sucrose medium in 2-1 flasks induced biphasic growth, with fructose as the only source of carbon and energy in the second phase.

引用

页码：77 / 82

页数：6

共 15 条

[1] INDUCTION OF STRESS METABOLITES IN IMMOBILIZED GLYCYRRHIZA-ECHINATA CULTURED-CELLS [J].