RNA EXPRESSION AND CHROMOSOMAL LOCATION OF THE MOUSE LONG-CHAIN ACYL-COA DEHYDROGENASE GENE

被引:11
作者
HINSDALE, ME
FARMER, SC
JOHNSON, KR
DAVISSON, MT
HAMM, DA
TOLWANI, RJ
WOOD, PA
机构
[1] UNIV ALABAMA, SCH MED, DEPT COMPARAT MED, BIRMINGHAM, AL 35294 USA
[2] UNIV ALABAMA, SCH DENT, BIRMINGHAM, AL 35294 USA
[3] JACKSON LAB, BAR HARBOR, ME 04609 USA
关键词
D O I
10.1006/geno.1995.1127
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The cDNA for mouse long-chain acyl-CoA dehydrogenase (Acadl, gene symbol; LCAD, enzyme) was cloned and characterized. The cDNA was obtained by library screening and reverse transcription-polymerase chain reaction (RT-PCR). The deduced amino acid sequence showed a high degree of homology to both the rat and the human LCAD sequence. Northern analysis of multiple tissues using the mouse Acadl cDNA as a probe showed two bands in all tissues examined. We found a total of three distinct mRNAs for Acadl. These three mRNAs were encoded by a single gene that we mapped to mouse chromosome 1. The three transcripts differed in the 3' untranslated region due to use of alternative polyadenylation sites. Quantitative evaluation of a multitissue Northern blot showed a varied ratio of the larger transcript as compared with the Smaller transcripts. (C) 1995 Academic Press, Inc.
引用
收藏
页码:163 / 170
页数:8
相关论文
共 39 条
[1]   A NOVEL DISEASE WITH DEFICIENCY OF MITOCHONDRIAL VERY-LONG-CHAIN ACYL-COA DEHYDROGENASE [J].
AOYAMA, T ;
UCHIDA, Y ;
KELLEY, RI ;
MARBLE, M ;
HOFMAN, K ;
TONSGARD, JH ;
RHEAD, WJ ;
HASHIMOTO, T .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 191 (03) :1369-1372
[2]  
BAKER EJ, 1993, CONTROL MESSENGER RN, P367
[3]   MULTIPLE MESSENGER-RNAS FOR HUMAN ALCOHOL-DEHYDROGENASE (ADH) - DEVELOPMENTAL AND TISSUE SPECIFIC DIFFERENCES [J].
BILANCHONE, V ;
DUESTER, G ;
EDWARDS, Y ;
SMITH, M .
NUCLEIC ACIDS RESEARCH, 1986, 14 (09) :3911-3926
[4]  
BREMER J, 1984, FATTY ACID METABOLIS, P113
[5]  
CARTER ME, 1993, J BIOL CHEM, V268, P13805
[6]   A PLEIOTROPIC ELEMENT IN THE MEDIUM-CHAIN ACYL-COENZYME A DEHYDROGENASE GENE PROMOTER MEDIATES TRANSCRIPTIONAL REGULATION BY MULTIPLE NUCLEAR RECEPTOR TRANSCRIPTION FACTORS AND DEFINES NOVEL RECEPTOR-DNA BINDING MOTIFS [J].
CARTER, ME ;
GULICK, T ;
MOORE, DD ;
KELLY, DP .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (07) :4360-4372
[7]   A GENETIC-LINKAGE MAP OF THE MOUSE - CURRENT APPLICATIONS AND FUTURE-PROSPECTS [J].
COPELAND, NG ;
JENKINS, NA ;
GILBERT, DJ ;
EPPIG, JT ;
MALTAIS, LJ ;
MILLER, JC ;
DIETRICH, WF ;
WEAVER, A ;
LINCOLN, SE ;
STEEN, RG ;
STEIN, LD ;
NADEAU, JH ;
LANDER, ES .
SCIENCE, 1993, 262 (5130) :57-66
[8]   IDENTIFICATION OF THE CATALYTIC BASE IN LONG-CHAIN ACYL-COA DEHYDROGENASE [J].
DJORDJEVIC, S ;
DONG, Y ;
PASCHKE, R ;
FRERMAN, FE ;
STRAUSS, AW ;
KIM, JJP .
BIOCHEMISTRY, 1994, 33 (14) :4258-4264
[9]   A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].
FEINBERG, AP ;
VOGELSTEIN, B .
ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13
[10]   PITUITARY-HORMONE FSH DIRECTS THE CREM FUNCTIONAL SWITCH DURING SPERMATOGENESIS [J].
FOULKES, NS ;
SCHLOTTER, F ;
PEVET, P ;
SASSONECORSI, P .
NATURE, 1993, 362 (6417) :264-267