KINETICS OF AN RNA CONFORMATIONAL SWITCH

被引:79
作者
LECUYER, KA [1 ]
CROTHERS, DM [1 ]
机构
[1] YALE UNIV,DEPT CHEM,NEW HAVEN,CT 06511
关键词
D O I
10.1073/pnas.91.8.3373
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The spliced leader RNA from Leptomonas collosoma has two competing secondary structures of nearly equal free energy. Short, complementary oligonucleotides can drive the structure from one form to the other. We report stopped-flow rapid-mixing and temperature-jump measurements of the kinetics of the structural switch. At high concentrations of oligonucleotide, the rate of binding becomes limited by the rate of the structural switch, which occurs on a time scale of a fraction of a second. The low activation energy observed for the process implies a branch migration type of mechanism in which portions of the two competing helices transiently coexist.
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收藏
页码:3373 / 3377
页数:5
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