AMPLIFIED BIOSENSING OF ALCOHOL BASED ON BIOCATALYTIC ACCUMULATION OF THE MEDIATOR

被引：12

作者：

WANG, J

NASER, N

机构：

[1] Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, New Mexico

来源：

ELECTROANALYSIS | 1995年 / 7卷 / 04期

关键词：

BIOSENSOR; BIOCATALYTIC ACCUMULATION; ALCOHOL; MELDOLA BLUE; ALCOHOL DEHYDROGENASE;

D O I：

10.1002/elan.1140070411

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel strategy for enhancing the sensitivity of alcohol-dehydrogenase amperometric biosensors, based on biocatalytic accumulation of the mediator, is described. By performing the biochemical/chemical step under open-circuit conditions, it is possible to accumulate the reduced form of the Meldola blue mediator, prior to the chronoamperometric quantitation of the enriched layer. Such temporal separation of the recognition and transduction events offers a 35-fold sensitivity enhancement (following a 5-min accumulation). Factors influencing the response are explored and applicability to various wine samples is illustrated. The new amplification strategy can be extended to biosensors based on other nicotinamide-cofactor dependent dehydrogenases.

引用

页码：362 / 364

页数：3

共 10 条

[1] ENZYMATIC DETERMINATION OF GLUCOSE IN A FLOW SYSTEM BY CATALYTIC-OXIDATION OF THE NICOTINAMIDE COENZYME AT A MODIFIED ELECTRODE [J].