THE EFFECT OF CALIBRATION ON THE BETWEEN-LABORATORY VARIATION OF SERUM FRUCTOSAMINE

被引:4
作者
HILL, RP
机构
关键词
D O I
10.1177/000456328802500422
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Human pooled serum from diabetic and non-diabetic subjects, and calibrators containing glycated protein of 1-deoxy-1-morpholinofructose (DMF) in a variety of matrices, were distributed to 10 laboratories. When they used their own assay conditions and calibrators, the inter-laboratory variation was unacceptably high. However, when the pool from diabetic patients was reassayed using a calibrator with an assigned value prepared from freeze-dried human serum, and containing no DMF, inter-laboratory variation was reduced significantly. Inter-laboratory agreement for the pool from non-diabetic subjects remained poor despite recalibration. Recalibration using either serum or albumin based solutions of DMF as calibrator failed to effect any significant reductions in inter-laboratory variation. Secondary calibrators based on a protein matrix with no added DMF are recommended for routine use.
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页码:435 / 439
页数:5
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