COMPARISONS OF T-CELL RECEPTOR (TCR) V-BETA REPERTOIRES OF LAMINA PROPRIA AND PERIPHERAL-BLOOD LYMPHOCYTES WITH RESPECT TO FREQUENCY AND OLIGOCLONALITY

被引:22
作者
AKOLKAR, PN
GULWANIAKOLKAR, B
MCKINLEY, M
FISHER, SE
SILVER, J
机构
[1] CORNELL UNIV MED COLL,N SHORE HOSP,DEPT MED,DIV GASTROENTEROL,MANHASSET,NY 11030
[2] CORNELL UNIV MED COLL,N SHORE HOSP,DEPT PEDIAT,MANHASSET,NY 11030
来源
CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY | 1995年 / 76卷 / 02期
关键词
D O I
10.1006/clin.1995.1110
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The TCR repertoires of CD4(+) and CD8(+) lamina propria and peripheral blood lymphocytes (PBL) were compared with respect to VP frequencies and oligoclonality. Disease-free colon specimens and paired peripheral blood samples were obtained from eight adult patients undergoing surgical resections for colorectal carcinoma. Mononuclear cells were isolated from the lamina propria and peripheral blood and separated into CD4(+) and CD8(+) cells by immunomagnetic adsorbtion. Analysis of VP frequencies by qPCR revealed that PBL and lamina propria lymphocytes (LPL) from the same individual have very different repertoires, especially within the CD8(+) population. Furthermore, CD8(+), but not CD4(+), LPL display extensive oligoclonality, similar to that which has previously been reported for CD8(+) PBL. However, the oligoclonal receptors observed in CD8(+) LPL are, in general, distinct from those observed in CD8(+) PBL, and differ for each individual. These observations indicate that the TCR repertoires of LPL are as diverse as PBL, and suggest that LPL and PBL are normally exposed to different sets of antigens. In addition, these observations provide a baseline for examining the effects of various disease states and environmental insults on the LPL repertoire. (C) 1995 Academic Press, Inc.
引用
收藏
页码:155 / 163
页数:9
相关论文
共 33 条
[1]  
AKOLKAR PN, 1993, J IMMUNOL, V150, P2761
[2]   THE EFFECTS OF MHC GENE DOSAGE AND ALLELIC VARIATION ON T-CELL RECEPTOR SELECTION [J].
BERG, LJ ;
FRANK, GD ;
DAVIS, MM .
CELL, 1990, 60 (06) :1043-1053
[3]   POSITIVE SELECTION OF CD4+T CELLS MEDIATED BY MHC CLASS-II-BEARING STROMAL CELL IN THE THYMIC CORTEX [J].
BILL, J ;
PALMER, E .
NATURE, 1989, 341 (6243) :649-651
[4]   THE ROLE OF THE T-CELL RECEPTOR IN POSITIVE AND NEGATIVE SELECTION OF DEVELOPING T-CELLS [J].
BLACKMAN, M ;
KAPPLER, J ;
MARRACK, P .
SCIENCE, 1990, 248 (4961) :1335-1341
[5]   INFLUENCE OF THE MAJOR HISTOCOMPATIBILITY COMPLEX ON POSITIVE THYMIC SELECTION OF V-BETA-17A+ T-CELLS [J].
BLACKMAN, MA ;
MARRACK, P ;
KAPPLER, J .
SCIENCE, 1989, 244 (4901) :214-217
[6]  
BLUMBERG RS, 1993, J IMMUNOL, V150, P5144
[7]   ISOLATION AND FUNCTIONAL CHARACTERIZATION OF HUMAN INTESTINAL MUCOSAL LYMPHOID-CELLS [J].
BULL, DM ;
BOOKMAN, MA .
JOURNAL OF CLINICAL INVESTIGATION, 1977, 59 (05) :966-974
[8]   INTERACTION OF STAPHYLOCOCCUS-AUREUS TOXIN SUPERANTIGENS WITH HUMAN T-CELLS [J].
CHOI, YW ;
KOTZIN, B ;
HERRON, L ;
CALLAHAN, J ;
MARRACK, P ;
KAPPLER, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (22) :8941-8945
[9]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[10]  
DAVEY MP, 1994, J IMMUNOL, V152, P315