USE OF S-ADENOSYLMETHIONINE AS AN INDEX OF METHIONINE RECYCLING IN RAT-LIVER SLICES

被引：18

作者：

BARAK, AJ

BECKENHAUER, HC

TUMA, DJ

机构：

[1] VET ADM MED CTR, LIVER STUDY UNIT, OMAHA, NE 68105 USA

[2] UNIV NEBRASKA, MED CTR, DEPT INTERNAL MED & BIOCHEM, OMAHA, NE 68105 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1982年 / 127卷 / 02期

关键词：

D O I：

10.1016/0003-2697(82)90189-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simplified method for the measurement of methionine recycling in liver slices was developed. The method, a double-labeling procedure, utilizes S-adenosylmethionine as an index of recycling and offers several advantages over methionine, which has been used in other laboratories to determine methionine recycling in plants and intact animals. These advantages include ease of isolation of index substance from tissue samples; elimination of oxidation problems prevalent with use of methionine; and need for less initial concentrations of [methyl-3H]methionine and [35S]methionine used as tracers in the measurement of methionine recycling.

引用

页码：372 / 375

页数：4

共 9 条

[1] NEW METHOD FOR ASSAY OF TISSUE S-ADENOSYLHOMOCYSTEINE AND S-ADENOSYLMETHIONINE - EFFECT OF PYRIDOXINE-DEFICIENCY ON METABOLISM OF S-ADENOSYLHOMOCYSTEINE, S-ADENOSYLMETHIONINE AND POLYAMINES IN RAT-LIVER [J].