CLONING OF A SPECIES-SPECIFIC DNA PROBE FROM ONCHOCERCA-GIBSONI

被引：6

作者：

GARATE, T ^{[1
]}

HARNETT, W ^{[1
]}

PARKHOUSE, RME ^{[1
]}

机构：

[1] NATL INST MED RES, DIV PARASITOL, LONDON NW7 1AA, ENGLAND

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1990年 / 20卷 / 01期

关键词：

Cloning; DNA probe; microfilaria; Onchocerca gibsoni;

D O I：

10.1016/0020-7519(90)90169-N

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A genomic library of Onchocerca gibsoni has been prepared in the vector lambda-gt10 and has been screened for specific DNA sequences by hybridization with radiolabelled total genomic DNA from a number of Onchocerca species. A clone-fOGl-has been isolated which does not interact with DNA prepared from O. gutturosa, O. lienalis, O. ochengi, O. cervicalis or O. volvulus (both Liberian and Mexican isolates). In addition, no hybridization is observed with host (cattle) DNA. fOGl can detect as little as 100-200 pg of O. gibsoni DNA. It is thus concluded that fOG 1 has the sensitivity to detect microfllariae of O. gibsoni found in the skin of cattle and the specificity to differentiate them from closely related species living in the same environment. © 1990.

引用

页码：31 / 35

页数：5

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