QUANTITATIVE EXTRACTION OF AMPHOTERICIN-B FROM SERUM AND ITS DETERMINATION BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY

被引:28
作者
BACH, PR [1 ]
机构
[1] CLMG INC, LOS ANGELES, CA 90057 USA
关键词
D O I
10.1128/AAC.26.3.314
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Therapeutic concentrations of amphotericin B [an antifungal drug having severe toxicity] in serum were measured by reversed-phase liquid chromatography with detection at 386 nm. Complete recovery of the drug and the internal standard from a 1-ml serum sample was achieved by pretreating the sample with guanidine hydrochloride and extracting it with a disposable reversed-phase phenyl extraction column. The method was sensitive to 0.005 .mu.g of amphotericin B per ml and linear to at least 5 .mu.g/ml. Coefficients of variation were 4.8% within-run and 6.3% between-run.
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页码:314 / 317
页数:4
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