Micropropagation of Anthyllis cytisoides (L) and Spartium junceum (L) 2 shrub legumes of ecological interest, was accomplished following standard procedures. Inoculation with the arbuscular mycorrhizal fungus Glomus fasciculatum, early during a post vitro weaning stage shortened the acclimatization process by 8 wk and particularly for Anthyllis improved survival, growth and nutrient status of microplants after outplanting. The level of mycorrhizal colonization of microplants was about the same as that for plants produced from seeds.