AN ELISA-METHOD USING MAGNETIC BEADS AS SOLID-PHASE FOR RAPID QUANTITATION OF MOUSE AND HUMAN-IMMUNOGLOBULINS

被引：7

作者：

RONNING, OW

CHRISTOPHERSEN, AC

机构：

[1] Center for Industrial Research, N-0314 Oslo 3

来源：

HYBRIDOMA | 1991年 / 10卷 / 05期

关键词：

D O I：

10.1089/hyb.1991.10.641

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This work describes an ELISA-method using superparamagnetic polystyrene beads (Dynabeads M-450) as solid phase, that may be used to measure the concentration of immunoglobulins in various fluids. The immunoglobulin was captured between beads coated with either rabbit, goat or sheep antibodies and the same antibody conjugated to horseradish peroxidase. The method has been used to quantitate mouse and human immunoglobulins in cell culture supernatants. Due to the rapid binding kinetcs between the beads and the soluble anigen (immunoglobulin), each incubation takes only ten minutes, allowing the whole assay to be performed within one hour.

引用

页码：641 / 645

页数：5

共 6 条

[1] MEASUREMENT OF THE CONCENTRATION OF MURINE IGG MONOCLONAL-ANTIBODY IN HYBRIDOMA SUPERNATANTS AND ASCITES IN ABSOLUTE UNITS BY SENSITIVE AND RELIABLE ENZYME-LINKED IMMUNOSORBENT ASSAYS (ELISA) [J].