PHOSPHOINOSITIDE SIGNALING ENZYMES IN RAT-LIVER NUCLEI - PHOSPHOINOSITIDASE-C ISOFORM BETA-1 IS SPECIFICALLY, BUT NOT PREDOMINANTLY, LOCATED IN THE NUCLEUS

被引：172

作者：

DIVECHA, N ^{[1
]}

RHEE, SG ^{[1
]}

LETCHER, AJ ^{[1
]}

IRVINE, RF ^{[1
]}

机构：

[1] NHLBI,BIOCHEM LAB,BETHESDA,MD 20892

来源：

BIOCHEMICAL JOURNAL | 1993年 / 289卷

关键词：

D O I：

10.1042/bj2890617

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The presence of phosphoinositide-mobilizing enzymes has been investigated in purified rat liver nuclei by radiolabelling and by probing with antibodies. A Ca2+-activated phosphoinositidase C (PIC) is present and was shown immunologically to be the beta1 isoform. No gamma- or delta-PIC was found. However, only 5% of the total beta1-PIC isoform is nuclear, with the majority being cytosolic. Galpha(q) and Galpha11 the suggested physiological activators of beta1-PIC, were not present. A Ptdlns4P 5-kinase is also present, which immunologically is shown to be the C isoform. All of these nuclear inositide enzymes still remained after the removal of the nuclear envelope with Triton X-100. consistent with the concept of an intranuclear inositide cycle [Divecha, Banfic and Irvine (1991) EMBO. J. 10, 3207-3214].

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页码：617 / 620

页数：4

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