HERPES-SIMPLEX VIRUS TYPE-1 CAPSID PROTEIN, VP21, ORIGINATES WITHIN THE UL26 OPEN READING FRAME

被引：46

作者：

PERSON, S

LAQUERRE, S

DESAI, P

HEMPEL, J

机构：

[1] Dept Molecular Genetics/Biochemistry, School of Medicine, Univ of Pittsburgh Medical Center, Pittsburgh

来源：

JOURNAL OF GENERAL VIROLOGY | 1993年 / 74卷

关键词：

D O I：

10.1099/0022-1317-74-10-2269

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The goal of experiments reported here was to identify the genes that encode capsid proteins VP21 and VP24 of herpes simplex virus type 1 (HSV-1). Capsids were isolated from infected cells and the proteins were separated by SDS-PAGE. N-terminal amino acid sequence analysis of partial CNBr digestion products. and of intact VP21, showed that it is encoded within the UL26 open reading frame (ORF) of HSV-1 beginning with codon 248 and probably extending to the end of the ORF (codon 635). Similar analysis of digestion products confirmed that VP24 is specified by codons 1 to 247 at the 5' end of the UL26 ORF. Each of the seven known capsid proteins has now been assigned to an ORF.

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页码：2269 / 2273

页数：5

相关论文

共 32 条

[1] 3-DIMENSIONAL STRUCTURES OF MATURABLE AND ABORTIVE CAPSIDS OF EQUINE HERPESVIRUS-1 FROM CRYOELECTRON MICROSCOPY [J].

BAKER, TS ;

NEWCOMB, WW ;

BOOY, FP ;

BROWN, JC ;

STEVEN, AC .

JOURNAL OF VIROLOGY, 1990, 64 (02) :563-573

[2] CHARACTERIZATION OF POST-TRANSLATIONAL PRODUCTS OF HERPES-SIMPLEX VIRUS GENE 35 PROTEINS BINDING TO THE SURFACES OF FULL CAPSIDS BUT NOT EMPTY CAPSIDS [J].

BRAUN, DK ;

ROIZMAN, B ;

PEREIRA, L .

JOURNAL OF VIROLOGY, 1984, 49 (01) :142-153

[3] STRUCTURAL-ANALYSIS OF THE CAPSID POLYPEPTIDES OF HERPES-SIMPLEX VIRUS TYPE-1 AND TYPE-2 [J].

COHEN, GH ;

PONCEDELEON, M ;

DIGGELMANN, H ;

LAWRENCE, WC ;

VERNON, SK ;

EISENBERG, RJ .

JOURNAL OF VIROLOGY, 1980, 34 (02) :521-531

[4] DIRECT DEMONSTRATION THAT THE ABUNDANT 6-KILOBASE HERPES-SIMPLEX VIRUS TYPE-1 MESSENGER-RNA MAPPING BETWEEN 0.23 AND 0.27 MAP UNITS ENCODES THE MAJOR CAPSID PROTEIN VP5 [J].

COSTA, RH ;

COHEN, G ;

EISENBERG, R ;

LONG, D ;

WAGNER, E .

JOURNAL OF VIROLOGY, 1984, 49 (01) :287-292

[5] DNA-SEQUENCE OF THE MAJOR CAPSID PROTEIN GENE OF HERPES-SIMPLEX VIRUS TYPE-1 [J].

DAVISON, AJ ;

SCOTT, JE .

JOURNAL OF GENERAL VIROLOGY, 1986, 67 :2279-2286

[6] IDENTIFICATION OF GENES ENCODING 2 CAPSID PROTEINS (VP24 AND VP26) OF HERPES-SIMPLEX VIRUS TYPE-1 [J].

DAVISON, MD ;

RIXON, FJ ;

DAVISON, AJ .

JOURNAL OF GENERAL VIROLOGY, 1992, 73 :2709-2713

[7] HERPES-SIMPLEX VIRUS TYPE-1 PROTEASE EXPRESSED IN ESCHERICHIA-COLI EXHIBITS AUTOPROCESSING AND SPECIFIC CLEAVAGE OF THE ICP35 ASSEMBLY PROTEIN [J].

DECKMAN, IC ;

HAGEN, M ;

MCCANN, PJ .

JOURNAL OF VIROLOGY, 1992, 66 (12) :7362-7367

[8] MUTATIONS IN HERPES-SIMPLEX VIRUS TYPE-1 GENES ENCODING VP5 AND VP23 ABROGATE CAPSID FORMATION AND CLEAVAGE OF REPLICATED DNA [J].

DESAI, P ;

DELUCA, NA ;

GLORIOSO, JC ;

PERSON, S .

JOURNAL OF VIROLOGY, 1993, 67 (03) :1357-1364

[9]

DILANNI CL, 1993, J BIOL CHEM, V268, P2048

[10] IDENTIFICATION OF PRECURSOR TO CYTOMEGALOVIRUS CAPSID ASSEMBLY PROTEIN AND EVIDENCE THAT PROCESSING RESULTS IN LOSS OF ITS CARBOXY-TERMINAL END [J].

GIBSON, W ;

MARCY, AI ;

COMOLLI, JC ;

LEE, J .

JOURNAL OF VIROLOGY, 1990, 64 (03) :1241-1249

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