GRAPHITE-TEFLON ENZYME ELECTRODE

被引：47

作者：

WANG, J

REVIEJO, AJ

ANGNES, L

机构：

[1] USP,INST QUIM,SAO PAULO,BRAZIL

[2] UNIV COMPLUTENSE MADRID,FAC CHEM,DEPT ANALYT CHEM,E-28040 MADRID,SPAIN

来源：

ELECTROANALYSIS | 1993年 / 5卷 / 07期

关键词：

BIOSENSOR; GRAPHITE TEFLON ELECTRODE; PEROXIDE; GLUCOSE;

D O I：

10.1002/elan.1140050708

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The fabrication and performance of graphite-Teflon-enzyme electrodes are described. The new bulk-modified composite enzyme electrodes couple the advantages of renewable (polishable) surfaces, mechanical rigidity, fast response, and favorable signal-to-noise characteristics. The new fabrication strategy is illustrated for the immobilization of horseradish peroxidase and glucose oxidase, in connection with measurements of peroxide species and glucose, respectively. The incorporation of a ferrocene mediator to yield a reagentless device is also illustrated. Flow injection measurements of hydrogen peroxide with the graphite-Teflon-peroxidase detector are carried out at 120 samples/hour, to yield a detection limit of 2.5 x 10(-6) M and a relative standard deviation of 2%. Applicability to organic-phase biosensing is illustrated.

引用

页码：575 / 579

页数：5

共 11 条

[1] AN AMPEROMETRIC GLUCOSE ELECTRODE BASED ON CARBON PASTE, CHEMICALLY MODIFIED WITH GLUCOSE-DEHYDROGENASE, NICOTINAMIDE ADENINE-DINUCLEOTIDE, AND A PHENOXAZINE MEDIATOR, COATED WITH A POLY(ESTER SULFONIC-ACID) CATION EXCHANGER [J].