PURIFICATION OF MEGAKARYOCYTE DIFFERENTIATION ACTIVITY FROM A HUMAN FIBROUS HISTIOCYTOMA CELL-LINE - N-TERMINAL SEQUENCE HOMOLOGY WITH ACTIVIN-A

被引：35

作者：

FUJIMOTO, K

KAWAKITA, M

KATO, K

YONEMURA, Y

MASUDA, T

MATSUZAKI, H

HIROSE, J

ISAJI, M

SASAKI, H

INOUE, T

TAKATSUKI, K

机构：

[1] KUMAMOTO UNIV,SCH MED,DEPT INTERNAL MED 2,1-1-1 HONJO,KUMAMOTO 860,JAPAN

[2] YOKOHAMA CITY UNIV,SCH MED,DEPT PATHOL,KANAZAWA KU,YOKOHAMA 236,JAPAN

[3] YOKOHAMA CITY UNIV,SCH MED,DEPT PEDIAT,KANAZAWA KU,YOKOHAMA 236,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 174卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)91543-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We purified a protein possessing a potent ability to induce the differentiation of a murine megakaryoblastic cell line, L8057, from the supernatant of a human fibrous histiocytoma cell line, KHM-5M. The protein, a homodimer of a molecular weight of 25-kDa, has an N-terminal sequence identical with that of the βA-chain of inhibin, indicating that it is identical to or highly homologous with activin A. Thus, it is speculated that activin A or its homologue is involved in megakaryocytic differentiation. © 1991.

引用

页码：1163 / 1168

页数：6

共 15 条

[1] QUANTITATION OF MEGAKARYOCYTOPOIESIS IN LIQUID CULTURE BY ENZYMATIC DETERMINATION OF ACETYLCHOLINESTERASE [J].