CLONING AND SEQUENCING OF THE GENE (TSG56) ENCODING A TYPE-SPECIFIC ANTIGEN FROM RICKETTSIA-TSUTSUGAMUSHI

被引：50

作者：

OHASHI, N

NASHIMOTO, H

IKEDA, H

TAMURA, A

机构：

[1] UNIV TOKYO, INST MED SCI, DEPT MOLEC BIOL, PO TAKANAWA, TOKYO 108, JAPAN

[2] NIIGATA COLL PHARM, DEPT MICROBIOL, NIIGATA 95021, JAPAN

[3] TEIKYO UNIV, DEPT BIOSCI, UTSUNOMIYA 320, JAPAN

来源：

GENE | 1990年 / 91卷 / 01期

关键词：

immunogenicity; obligate intracellular parasite; Recombinant DNA; scrub typhus; signal peptide; transmembrane protein;

D O I：

10.1016/0378-1119(90)90171-M

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A type-specific antigen (TSA) in Rickettsia tsutsugamushi is a 56-kDa protein located on the rickettsial surface. The gene (tsg56) encoding the TSA was isolated from R. tsutsugamushi Gilliam strain. Sequencing revealed an open reading frame of 1572 bp encoding a 524-amino acid (aa) protein with an Mr of 56 053. The deduced as sequence consists of a 22-aa signal sequence and the mature TSA sequence (502 aa; Mr 53 803), whose N-terminal portion has the aa sequence determined directly in our previous study [Ohashi et al., Infect. Immun. 57 (1989) 1427-1431]. Alternate localization of hydrophobic and hydrophilic regions was recognized in the TSA molecule, suggesting that the TSA is a transmembrane protein. The codon usage in the A + T-rich (59%) tsg56 gene reflects a high A + T content of R. tsutsugamushi. The 5′-flanking region contains sequences similar to those of ribosome-binding sites and promoters of Escherichia coli. © 1990.

引用

页码：119 / 122

页数：4

共 21 条

[1] IDENTIFICATION BY SEQUENCE-ANALYSIS OF 2-SITE POSTTRANSLATIONAL PROCESSING OF THE CYSTEINE-RICH OUTER-MEMBRANE PROTEIN-2 OF CHLAMYDIA-TRACHOMATIS SEROVAR-L2 [J].