INDUCTION OF ANTIBACTERIAL PROTEIN-SYNTHESIS BY SOLUBLE PEPTIDOGLYCAN IN ISOLATED FAT-BODY FROM LARVAE OF THE SILKWORM, BOMBYX-MORI

被引：29

作者：

IKETANI, M ^{[1
]}

MORISHIMA, I ^{[1
]}

机构：

[1] TOTTORI UNIV, FAC AGR, METAB BIOCHEM LAB, TOTTORI 680, JAPAN

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1993年 / 23卷 / 08期

关键词：

BOMBYX-MORI; ANTIBACTERIAL PROTEIN; CECROPIN; LYSOZYME; PEPTIDOGLYCAN; FAT BODY IN-VITRO INDUCTION; INSECT IMMUNITY;

D O I：

10.1016/0965-1748(93)90108-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Synthesis and secretion of bactericidal protein (cecropin) and.lysozyme were induced by soluble peptidoglycan fragments (SPG) from Escherichia coli in a culture of fat body from Bombyx mori larvae. The rate of the secretion by fat body increased as a function of SPG concentration added to the culture medium. The induction of bactericidal activity was specific for peptidoglycan of a particular structure. Thus, SPG from Micrococcus luteus was 500-times less potent than E. coli SPG, and various glucans and peptides structurally related to peptidoglycan were all ineffective as elicitor. These results support the hypothesis that bacteria invading the haemocoel have to be partially degraded to generate peptidoglycan fragments as a signal molecule, which subsequently acts on a receptor on fat body cells and induces antibacterial protein synthesis.

引用

页码：913 / 917

页数：5

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