A SIMPLE AND RAPID METHOD FOR THE PURIFICATION OF GROEL, AN ESCHERICHIA-COLI HOMOLOG OF THE HEAT-SHOCK PROTEIN 60 FAMILY OF MOLECULAR CHAPERONINS

被引:12
作者
KHANDEKAR, SS
BETTENCOURT, BM
KELLEY, KC
RECNY, MA
机构
[1] Procept, Inc., Cambridge, MA 02139
关键词
D O I
10.1006/prep.1993.1076
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
GroEL, an Escherichia coli homolog of the heat shock protein 60 family of molecular chaperonins, has been implicated as a target of T cell-mediated immune responses in a broad spectrum of infections. In order to produce large quantities of native protein for raising and stimulating GroEL specific T cell lines, we have developed a simple and rapid two-step protocol for purifying native E. coli GroEL heat shock (or stress) protein which takes advantage of the inherent structural and functional properties of the protein. Based on a combination of gel exclusion chromatography, ATPase activity assay, isoelectric focusing, and circular dichroism analyses we conclude that our purification process yields native tetradecameric GroEL. © 1993 Academic Press. All rights reserved.
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页码:580 / 584
页数:5
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