ISOLATION AND CHARACTERIZATION OF DNA-SEQUENCES FROM FLOW-SORTED HUMAN-CHROMOSOME 22 LIBRARIES

被引：5

作者：

METZDORF, R ^{[1
]}

HERZOG, R ^{[1
]}

GOTTERT, E ^{[1
]}

BLIN, N ^{[1
]}

机构：

[1] UNIV SAARLAND,DEPT HUMAN GENET,W-6650 HOMBURG,GERMANY

来源：

HUMAN HEREDITY | 1990年 / 40卷 / 03期

关键词：

chromosome; 22; DNA libraries; DNA polymorphism; mapping;

D O I：

10.1159/000153922

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Two flow-sorted chromosome 22 libraries were used to isolate DNA sequences specific for chromosome 22. 45-phage DNAs were probed against human genomic DNA. 12 of them showed unique or low-copycharacter. Using digested DNA from rodent-human hybrid cell lines, 3 of the 12 recombinants were assigned unique to chromosome 22 and regionally mapped. 1 clone mapped to 22pter-q11, 1 clone to 22q12-qter and 1 clone, for which in situ hybridization was performed, to 22q13.1. 2 low-copy probes, 1 of them displaying polymorphisms in MspI and TaqI digests of individual DNAs, must have similar sequences on 22 and additional chromosomes. Furthermore, a highly repetitive DNA representing a compound locus of some hundred kilobases on chromosome 22 was isolated. These 6 probes may provide useful tools for studying the structure and function of this small chromosome involved in a relatively high number of inherited and acquired diseases.

引用

页码：145 / 152

页数：8

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