IMMUNOELECTRON MICROSCOPIC LOCALIZATION OF THYROTROPIN-RELEASING-HORMONE (TRH) PRECURSOR IN THE RAT RAPHE NUCLEI

被引：18

作者：

LIAO, N

BULANT, M

NICOLAS, P

VAUDRY, H

PELLETIER, G

机构：

[1] UNIV LAVAL,MED CTR,MRC,MOLEC ENDOCRINOL GRP,2705 LAURIER BLVD,QUEBEC CITY G1V 4G2,QUEBEC,CANADA

[2] UNIV ROUEN HAUTE NORMANDIE,ENDOCRINOL MOLEC LAB,CNRS,UA 650,INSERM,UNITE ALLIEE,F-76130 MT ST AIGNAN,FRANCE

来源：

PEPTIDES | 1990年 / 11卷 / 02期

关键词：

Immunocytochemistry; Raphe nuclei; Rat brain; Thyrotropin-releasing hormone;

D O I：

10.1016/0196-9781(90)90099-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using antisera to two pro-thyrotropin-releasing hormone (pro-TRH)-derived cryptic peptides, we have studies by immunocytochemistry the ultrastructural localization of pro-TRH in the rat raphe nuclei. The same results were obtained with both antisera. Immunostaining was found in cell bodies, dendrites and endings. In cell bodies, the reaction product was restricted to Golgi saccules and dense core vesicles which were very few in number. In dendrites, the staining was rather diffuse without any association with specific organelles. These results suggest that the Golgi apparatus might be involved in pro-TRH processing. © 1990.

引用

页码：397 / 400

页数：4

共 9 条

[1]

CHRETIEN M, 1984, CELL BIOL SECRETORY, P214

[2] FURTHER-STUDIES ON THE IDENTIFICATION OF NEURONS CONTAINING IMMUNOREACTIVE ALPHA-MELANOCYTE-STIMULATING HORMONE (ALPHA-MSH) IN THE RAT-BRAIN [J].