ELASTASE DIGESTION OF NORMAL AND PSEUDOXANTHOMA ELASTICUM LESIONAL SKIN ELASTINS

被引:5
作者
SCHWARTZ, E
THIEBERG, M
CRUICKSHANK, FA
LEBWOHL, M
机构
[1] Department of Dermatology, Mount Sinai School of Medicine, New York
关键词
D O I
10.1016/0014-4800(91)90052-Y
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Pseudoxanthoma elasticum (PXE) is a heritable disorder of connective tissue that is characterized by redundant folds of skin in flexural areas. There is considerable evidence that suggests that the elastic fiber is the main site of the abnormality although the primary molecular defect has not been identified. The aim of this study was to identify differences between PXE and normal skin elastins. Elastins from normal, nonsolar-exposed skin, and pseudoxanthoma elasticum lesional skin were purified and their solubilization by pancreatic elastase was compared. Results demonstrated that elastin derived from normal skin was more susceptible to proteolytic cleavage than elastin purified from either pseudoxanthoma elasticum lesional skin or ligamentum nuchae. Pretreatment of the lesional elastin with testicular hyaluronidase increased its solubilization two-fold and generated a unique 15,000 Da molecular weight fragment. Elastin prepared from PXE skin may contain bound glycosaminoglycans which interfere with elastase activity. The susceptibility of normal skin elastin to proteolytic degradation may have implications in the study of aging skin. © 1991.
引用
收藏
页码:190 / 195
页数:6
相关论文
共 17 条
[1]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[2]   MORPHOLOGIC CHANGES IN PSEUDOXANTHOMA ELASTICUM AND SENILE SKIN [J].
DANIELSEN, L .
INTERNATIONAL JOURNAL OF DERMATOLOGY, 1982, 21 (08) :449-454
[3]   LYSYL OXIDASE ACTIVITY AND ELASTIN GLYCOSAMINOGLYCAN INTERACTIONS IN GROWING CHICK AND RAT AORTAS [J].
FORNIERI, C ;
BACCARANICONTRI, M ;
QUAGLINO, D ;
PASQUALIRONCHETTI, I .
JOURNAL OF CELL BIOLOGY, 1987, 105 (03) :1463-1469
[4]   STRUCTURE OF THE ELASTIN GENE AND ALTERNATIVE SPLICING OF ELASTIN MESSENGER-RNA - IMPLICATIONS FOR HUMAN-DISEASE [J].
INDIK, Z ;
YEH, H ;
ORNSTEINGOLDSTEIN, N ;
KUCICH, U ;
ABRAMS, W ;
ROSENBLOOM, JC ;
ROSENBLOOM, J .
AMERICAN JOURNAL OF MEDICAL GENETICS, 1989, 34 (01) :81-90
[5]   PROTEOLYSIS OF ELASTIN-LIGAND COMPLEXES - STIMULATION OF ELASTASE DIGESTION OF INSOLUBLE ELASTIN BY SODIUM DODECYL SULFATE [J].
KAGAN, HM ;
JORDAN, RE ;
CROMBIE, GD ;
LEWIS, W ;
FRANZBLA.C .
BIOCHEMISTRY, 1972, 11 (18) :3412-&
[6]  
KOREEN R, 1987, J INVEST DERMATOL, V88, P500
[7]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[8]   GLYCOSAMINOGLYCANS OF SKIN AND URINE IN PSEUDOXANTHOMA ELASTICUM - EVIDENCE FOR CHONDROITIN 6-SULFATE ALTERATION [J].
LONGAS, MO ;
WISCH, P ;
LEBWOHL, MG ;
FLEISCHMAJER, R .
CLINICA CHIMICA ACTA, 1986, 155 (03) :227-236
[9]  
MARTINEZ.A, 1974, LAB INVEST, V31, P181
[10]  
MCKUSICK VA, 1972, HERITABLE DISORDERS, P475