AN INTERVENING SEQUENCE (IVS) IN THE 16S RIBOSOMAL-RNA GENE OF THE EUBACTERIUM HELICOBACTER-CANIS

PCR amplicons enlarged by similar to 250bp were generated from the 16S rRNA (rrs) genes of certain strains of the recently described Helicobacter species, H.canis. The DNA sequence of the rrs gene of one such strain was determined, and it was shown that an intervening sequence (IVS) of 235bp followed nucleotide 199 in the rrs sequence. In four other H.canis strains, identical or similar IVSs were found, always at the same location in the rrs gene. The secondary structures of the RNA transcripts of the IVSs were predicted. They were characterised by the presence of a conserved stem-loop structure, a potential recognition site for RNA processing enzymes. Ribosomal RNA was compared from a strain of H.canis with and without the IVS-containing rrs gene. In the former 16S rRNA appeared as two fragments, whose sizes were consistent with cleavage at either side of the IVS, and which were not subsequently religated. The IVS sequence was not represented elsewhere in the H.canis genome. Its evolutionary significance is discussed.