ENZYME OR PROTEIN IMMOBILIZATION TECHNIQUES FOR APPLICATIONS IN BIOSENSOR DESIGN

被引:206
作者
SCOUTEN, WH [1 ]
LUONG, JHT [1 ]
BROWN, RS [1 ]
机构
[1] NATL RES COUNCIL CANADA, BIOTECHNOL RES INST, MONTREAL, PQ H4P 2R2, CANADA
关键词
D O I
10.1016/S0167-7799(00)88935-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
New generations of biosensors are emerging that are based on novel and promising transducers such as miniature, reagentless-mediated electrodes, field-effect transistors, piezoelectric and optical devices, Reagentless-mediated biosensors can be constructed by co-immobilizing both enzymes and mediators onto a miniaturized electrode using electropolymerization, thus improving the sensitivity and speed of the response, Even more promising is the development of electrochemical sensors, in which electron transfer is made directly from a redox enzyme to an electrode surface via molecular wires. While this has only been reported, so far, for a specific enzyme entrapped in N-methylpyrrole under defined circumstances, the development of new oriented immobilization techniques, coupled with progress in protein engineering, may make direct electron transfer the rule rather than the exception.
引用
收藏
页码:178 / 185
页数:8
相关论文
共 47 条
[1]   AN OPTICAL CHEMICAL SENSOR USING A FLUOROPHORE-EMBEDDED LANGMUIR-BLODGETT FILM [J].
AIZAWA, M ;
MATSUZAWA, M ;
SHINOHARA, H .
THIN SOLID FILMS, 1988, 160 (1-2) :477-481
[2]  
ALENXANDRE S, 1994, MICROSC MICROANAL M, V5, P61
[3]  
BARTLETT PN, 1993, J ELECTROANAL CHEM, V362, P1
[4]   Built-up films of barium stearate and their optical properties [J].
Blodgett, KB ;
Langmuir, I .
PHYSICAL REVIEW, 1937, 51 (11) :0964-0982
[5]   FLUORESCENCE TRANSDUCTION OF AN ENZYME SUBSTRATE REACTION BY MODULATION OF LIPID-MEMBRANE STRUCTURE [J].
BRENNAN, JD ;
BROWN, RS ;
MCCLINTOCK, CP ;
KRULL, UJ .
ANALYTICA CHIMICA ACTA, 1990, 237 (02) :253-263
[6]  
Chibata I, 1978, IMMOBILIZED ENZYMES
[7]   IMMOBILIZATION OF PROTEIN-A AT HIGH-DENSITY ON AZLACTONE-FUNCTIONAL POLYMERIC BEADS AND THEIR USE IN AFFINITY-CHROMATOGRAPHY [J].
COLEMAN, PL ;
WALKER, MM ;
MILBRATH, DS ;
STAUFFER, DM ;
RASMUSSEN, JK ;
KREPSKI, LR ;
HEILMANN, SM .
JOURNAL OF CHROMATOGRAPHY, 1990, 512 :345-363
[8]  
COULET PR, 1988, Patent No. 23283
[9]   SOL-GEL ENCAPSULATION METHODS FOR BIOSENSORS [J].
DAVE, BC ;
DUNN, B ;
VALENTINE, JS ;
ZINK, JI .
ANALYTICAL CHEMISTRY, 1994, 66 (22) :A1120-A1127
[10]  
DUPOET PD, 1990, ANAL CHIM ACTA, V235, P225