CATALYTIC ACTIVITY OF ASPERGILLUS-NIGER GLUCOSE-OXIDASE IN WATER-POLYOL MIXTURES

被引：5

作者：

CIOCI, F

机构：

[1] Department of Chemical Engineering, University 'LaSapienza', Rome, I-00184

来源：

CATALYSIS LETTERS | 1995年 / 35卷 / 3-4期

关键词：

GLUCOSE OXIDASE; CATALYTIC ACTIVITY; PROTEIN UNFOLDING; WATER-MISCIBLE SOLVENTS;

D O I：

10.1007/BF00807196

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

An experimental investigation was carried out on the kinetic and stability behaviour of Aspergillus niger glucose oxidase in water-polyol mixtures. Kinetic runs performed in systems containing up to 8 M polyhydric cosolvents (ethylene glycol, propylene glycol and glycerol) showed a strong dependence of the catalytic properties of the enzyme on the nature and composition of the reaction medium. Higher residual activities were measured in the presence of additives of lower polarity, according to the following order: propylene glycol > ethylene glycol > glycerol. Dilution and thermal unfolding experiments provided evidence that the activity reductions were not ascribable to solvent-induced structural modifications of the enzyme molecule but, rather, to the inhibitory action exerted by the polyols.

引用

页码：395 / 405

页数：11

共 28 条

[1] PREFERENTIAL INTERACTIONS OF PROTEINS WITH SOLVENT COMPONENTS IN AQUEOUS AMINO-ACID SOLUTIONS [J].