METHYLATION PATTERNS AT THE HYPERVARIABLE X-CHROMOSOME LOCUS DXS255 (M27-BETA) - CORRELATION WITH X-INACTIVATION STATUS

被引：123

作者：

BOYD, Y ^{[1
]}

FRASER, NJ ^{[1
]}

机构：

[1] UNIV OXFORD,DEPT BIOCHEM,OXFORD OX1 3QU,ENGLAND

来源：

GENOMICS | 1990年 / 7卷 / 02期

关键词：

D O I：

10.1016/0888-7543(90)90539-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Methylation patterns surrounding a hypervariable X-chromosome locus, DXS255, have been analyzed with the restriction enzyme MspI and its methylation-sensitive isoschizomer HpaII. HpaII sites flanking the hypervariable region were found to be methylated on 41 active X chromosomes preuunmethylated on 11 inactive X chromosomes present in a range of male, female, and hybrid cells and tissues. This differential methylation pattern coupled with the previously described high level (>90%) of heterozygosity at the DXS255 locus can therefore be applied to determine the inactivation status of X chromosomes in females heterozygous for X-linked disease and in tumor clonality studies. © 1990.

引用

页码：182 / 187

页数：6

共 25 条

[1] CPG-RICH ISLANDS AND THE FUNCTION OF DNA METHYLATION [J].