INTENSIFICATION OF PEROXIDASE-DIAMINOBENZIDINE STAINING USING GOLD-SULFIDE-SILVER - A RAPID AND HIGHLY SENSITIVE METHOD FOR VISUALIZATION IN IMMUNOBLOTTING

被引：13

作者：

IIDA, R ^{[1
]}

YASUDA, T ^{[1
]}

NADANO, D ^{[1
]}

KISHI, K ^{[1
]}

机构：

[1] FUKUI UNIV, DEPT LEGAL MED, MATSUOKA CHO, FUKUI 910, JAPAN

来源：

ELECTROPHORESIS | 1990年 / 11卷 / 10期

关键词：

D O I：

10.1002/elps.1150111014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A highly sensitive and rapid visualization method for protein detection by immunoblotting is described. Proteins blotted onto a Durapore membrane were visualized by the following procedure: after conventional peroxidase‐based staining with 3,3′‐diaminobenzidine (DAB), the produced DAB precipitates were intensified by treating with (i) gold trichloride (acid), (ii) sodium sulfide, and (iii) a developer containing silver nitrate. This postintensification method was employed for the detection of the genetic polymorphism of human proteins, such as deoxyribonuclease I in urine, and group specific component, transferrin and α1‐antitrypsin in serum after polyacrylamide gel‐isoelectric focusing, followed by immunoblotting. This postintensification technique was found to be simple, giving up to 16‐ to 64‐fold amplification of the conventional peroxidase‐DAB staining. Copyright © 1990 VCH Verlagsgesellschaft mbH

引用

页码：852 / 855

页数：4

共 23 条

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