SATURATION KINETICS OF PALMITATE UPTAKE IN PERFUSED SKELETAL-MUSCLE

被引：91

作者：

TURCOTTE, LP

KIENS, B

RICHTER, EA

机构：

来源：

FEBS LETTERS | 1991年 / 279卷 / 02期

关键词：

FREE FATTY ACID; MUSCLE UPTAKE;

D O I：

10.1016/0014-5793(91)80180-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the kinetics of palmitate uptake in a physiological skeletal muscle preparation by using the isolated perfused rat hindquarter. When plotted against the unbound plasma palmitate concentration, palmitate uptake displayed a simple Michaelis-Menten relation with a calculated V(max) and K(m) of 16.3 nmol.min-1.g-1 and 0.06-mu-mol.1(-1), respectively. These results show that, as in isolated cell systems, uptake of free fatty acids in perfused skeletal muscle follows saturation kinetics consistent with carrier-mediated membrane transport of free fatty acids.

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页码：327 / 329

页数：3

相关论文

共 16 条

[1] REGULATION OF PLASMA FREE FATTY ACID TURNOVER [J].

ARMSTRONG, D ;

STEELE, R ;

DEBODO, RC ;

DUNN, A ;

BISHOP, JS ;

ALTSZULER, N .

AMERICAN JOURNAL OF PHYSIOLOGY, 1961, 201 (01) :9-&

[2] SIMULTANEOUS ANALYSIS OF FAMILIES OF SIGMOIDAL CURVES - APPLICATION TO BIOASSAY, RADIOLIGAND ASSAY, AND PHYSIOLOGICAL DOSE-RESPONSE CURVES [J].

DELEAN, A ;

MUNSON, PJ ;

RODBARD, D .

AMERICAN JOURNAL OF PHYSIOLOGY, 1978, 235 (02) :E97-E102

[3] RELATION BETWEEN NON-ESTERIFIED FATTY ACIDS IN PLASMA AND THE METABOLISM OF GLUCOSE [J].

DOLE, VP .

JOURNAL OF CLINICAL INVESTIGATION, 1956, 35 (02) :150-154

[4] TURNOVER RATE OF PLASMA FFA IN HUMANS AND IN DOGS [J].

ISSEKUTZ, B ;

BORTZ, WM ;

MILLER, HI ;

PAUL, P .

METABOLISM-CLINICAL AND EXPERIMENTAL, 1967, 16 (11) :1001-&

[5] SEPARATION WITH UNI-DIMENSIONAL TLC OF ALL NEUTRAL LIPID CLASSES [J].

KELLEY, TF .

JOURNAL OF CHROMATOGRAPHY, 1966, 22 (02) :456-&

[6]

KIENS B, 1984, THESIS U COPENHAGEN

[7]

MILLER WC, 1988, P SOC EXP BIOL MED, V189, P183

[8] MECHANISMS LIMITING GLYCOGEN-STORAGE IN MUSCLE DURING PROLONGED INSULIN STIMULATION [J].

RICHTER, EA ;

HANSEN, SA ;

HANSEN, BF .

AMERICAN JOURNAL OF PHYSIOLOGY, 1988, 255 (05) :E621-E628

[9] EVALUATION OF ISOLATED PERFUSED RAT HINDQUARTER FOR STUDY OF MUSCLE METABOLISM [J].

RUDERMAN, NB ;

HOUGHTON, CR ;

HEMS, R .

BIOCHEMICAL JOURNAL, 1971, 124 (03) :639-&

[10] UPTAKE OF OLEATE BY ISOLATED RAT ADIPOCYTES IS MEDIATED BY A 40-KDA PLASMA-MEMBRANE FATTY-ACID BINDING-PROTEIN CLOSELY RELATED TO THAT IN LIVER AND GUT [J].

SCHWIETERMAN, W ;

SORRENTINO, D ;

POTTER, BJ ;

RAND, J ;

KIANG, CL ;

STUMP, D ;

BERK, PD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (02) :359-363