DIRECT RESOLUTION OF OPTICALLY-ACTIVE ISOMERS ON CHIRAL PACKINGS CONTAINING ERGOLINE SKELETONS .2. ENANTIOSEPARATION OF CARBOXYLIC-ACIDS

Structurally different carboxylic acids and dansyl derivatives of amino acids were examined on a chiral stationary phase for high-performance liquid chromatography. The packing was prepared by bonding to silica gel an aminopropyl derivative of the ergot alkaloid (+)-terguride. Optimization of the enantioseparations was attained through the study of the influence of the organic modifier content and the pH and ionic strength of the buffer in the eluent. Electrostatic and hydrophobic interactions between the ergot alkaloid and the analyte contribute to a large extent to the retention and chiral discrimination. The selector was shown to be effective for the resolution of dicarboxylic acids, 2-arylcarboxylic acids and amino acid derivatives.