MOLECULAR-CLONING AND PARTIAL CHARACTERIZATION OF A NOVEL COLLAGEN CHAIN, ALPHA-1(XVI), CONSISTING OF REPETITIVE COLLAGENOUS DOMAINS AND CYSTEINE-CONTAINING NONCOLLAGENOUS SEGMENTS

被引:31
作者
YAMAGUCHI, N
KIMURA, S
MCBRIDE, OW
HORI, H
YAMADA, Y
KANAMORI, T
YAMAKOSHI, H
NAGAI, Y
机构
[1] TOKYO MED & DENT UNIV,MED RES INST,DEPT TISSUE PHYSIOL,2-3-10 KANDASURUGADAI,CHIYODA KU,TOKYO 101,JAPAN
[2] NCI,MOLEC CARCINOGENESIS LAB,BETHESDA,MD 20892
[3] NCI,BIOCHEM LAB,BETHESDA,MD 20892
[4] NIDR,DEV BIOL & ANOMALIES LAB,BETHESDA,MD 20892
关键词
D O I
10.1093/oxfordjournals.jbchem.a123989
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In an effort to identify new members of the collagen family, we screened a human placenta cDNA library with a collagenous probe. A novel 3.7 kb cDNA was identified encoding an open reading frame of 1, 186 amino acids and containing a termination codon. The predicted polypeptide consists of 9 repetitive collagenous (stretches of Gly-X-Y) and several non-collagenous segments. Two cysteinyl residues separated by two amino acid residues (Cys-X-X-Cys) are regularly located in the N-terminal region of each non-collagenous segment. The deduced amino acid sequence described above is distinct from those of known types of collagen. Therefore, this novel collagen chain is designated alpha1(XVI). Northern blot analysis revealed an alpha1(XVI) mRNA of 5.2 kb, indicating that the overlapping cDNA clones isolated in this study covered nearly three-fourths of the mRNA. As a tool for further study on the expression of type XVI collagen, we prepared an antibody against the nonadecapeptide CFLSLERPRAEEARGDNSE, derived from the putative translation product of the cDNA. In immunoblot analysis, the antibody recognized a 160 kDa protein, which was bacterial collagenase-sensitive. Immunohistochemical stainings of human placental tissues with anti-peptide antibody revealed a positive reaction with amnion, the membranous tissue lining the amniotic cavity. The gene of alpha1(XVI) chain, COL16A1, is mapped on the short arm of human chromosome 1(1p13-p34).
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页码:856 / 863
页数:8
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