IMMUNOASSAY USING A METAL-COMPLEX COMPOUND AS A CHEMI-LUMINESCENT CATALYST .2. AN IMPROVEMENT OF THE ANALYTICAL METHOD FOR PRACTICAL USE

Immunoassay using a synthesized metal-complex compound as a labeling reagent has been investigated. Iron(III) 2,9,16,23-tetrakis(chlorocarbonyl)phthalocyanine, which showed a marked catalytic action in the chemiluminescence reaction between luminol and H//2O//2, was synthesized, and albumin was labeled with it. Albumin in the range from 50 ng/tube to 50 mu g/tube could be determined by measuring the chemiluminescence intensity in a competitive immunoassay using glass beads immobilized with an antibody. The present method became about 40 times as sensitive as the previous method by optimizing the labeling procedure, the chemiluminescence reaction, and the immune reaction.