SEQUENTIAL ASSIGNMENT OF THE H-1 NUCLEAR-MAGNETIC-RESONANCE SPECTRUM OF BARNASE

被引:39
作者
BYCROFT, M [1 ]
SHEPPARD, RN [1 ]
LAU, FT [1 ]
FERSHT, AR [1 ]
机构
[1] IMPERIAL COLL SCI TECHNOL & MED,DEPT CHEM,LONDON SW7 2AY,ENGLAND
关键词
D O I
10.1021/bi00484a011
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two-dimensional nuclear magnetic resonance spectroscopy has been used to study the bacterial ribonuclease barnase (MW 12 382). Resonance assignments have been made for protons in all of the 110 residues. Analysis of medium- and long-range contacts in NOESY spectra has demonstrated that the major elements of secondary structure in barnase in solution are essentially identical with those found in the crystal structure. © 1990, American Chemical Society. All rights reserved.
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页码:7425 / 7432
页数:8
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