DISC-CROSSED IMMUNOELECTROPHORESIS - SIMPLE LAYING-ON TECHNIQUE PERMITTING USE OF COMMERCIALLY AVAILABLE AGAROSE

被引：15

作者：

EKWALL, K ^{[1
]}

SODERHOLM, J ^{[1
]}

WADSTROM, T ^{[1
]}

机构：

[1] STATENS BAKTERIOL LAB, S-10521 STOCKHOLM, SWEDEN

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1976年 / 12卷 / 1-2期

关键词：

D O I：

10.1016/0022-1759(76)90100-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A technique, disc-crossed immunoelectrophoresis, is described to provide a simple and convenient means of performing electrophoresis in polyacrylamide gel in a discontinuous buffer system (disc electrophoresis) followed by electrophoresis into antibody [horse diphtheria antitoxin, rabbit antihuman transferrin]-containing agarose gel. A simple washing of the polyacrylamide gel in distilled water combined with a laying-on method, seems to be a satisfactory and rapid way to obtain immunoprecipitation patterns of high quality using commercially available agarose in the 2nd electrophoresis.

引用

页码：103 / 115

页数：13

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