TRANSPOSON TN5SUPF-BASED REVERSE GENETIC METHOD FOR MUTATIONAL ANALYSIS OF ESCHERICHIA-COLI WITH DNAS CLONED IN LAMBDA-PHAGE

被引：10

作者：

PHADNIS, SH

KULAKAUSKAS, S

KRISHNAN, BR

HIEMSTRA, J

BERG, DE

机构：

[1] WASHINGTON UNIV, SCH MED, DEPT GENET, BOX 8230, ST LOUIS, MO 63110 USA

[2] WASHINGTON UNIV, SCH MED, DEPT MOLEC MICROBIOL, ST LOUIS, MO 63110 USA

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 02期

关键词：

D O I：

10.1128/jb.173.2.896-899.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An efficient method for systematic mutational analysis of the Escherichia coli genome was developed. It entails Tn5supF transposition to lambda-E. coli hybrid phage clones (Kohara library) and then transduction of recipient cells to Sup+. Essential and nonessential genes are distinguished by the ability of insertion mutant phage to form haploid versus only heterozygous partial diploid bacterial recombinants.

引用

收藏

页码：896 / 899

页数：4

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