TRANSACTIVATION IN A GEMINIVIRUS - AL2 GENE-PRODUCT IS NEEDED FOR COAT PROTEIN EXPRESSION

被引：132

作者：

SUNTER, G

BISARO, DM

机构：

[1] OHIO STATE UNIV,CTR BIOTECHNOL,COLUMBUS,OH 43210

[2] OHIO STATE UNIV,DEPT MOLEC GENET,COLUMBUS,OH 43210

来源：

VIROLOGY | 1991年 / 180卷 / 01期

关键词：

D O I：

10.1016/0042-6822(91)90049-H

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The β-glucuronidase (GUS) reporter gene was used to replace the coat protein gene (open reading frame AR1) of tomato golden mosaic virus (TGMV) and transiently expressed in tobacco protoplasts. While these TGMV/GUS genomes gave a high level of GUS activity, genomes which also contained a mutation in the AL 2 open reading frame (TGMV/GUS/AL2-) did not express GUS. GUS activity could be restored by cotransfecting protoplasts with the TGMV/GUS/AL2- genome and a wild-type TGMV genome. Thus, the AL2 gene product transactivates expression of TGMV coat protein gene. © 1991.

引用

页码：416 / 419

页数：4

共 20 条

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