POLYLYSINE ACTIVATES MEMBRANE-BOUND ADENYLYL CYCLASE FROM XENOPUS-LAEVIS OOCYTES THROUGH THE GS TRANSDUCING PROTEIN

被引：11

作者：

ANTONELLI, M

OLATE, J

ALLENDE, CC

ALLENDE, JE

机构：

[1] UNIV CHILE,FAC MED,DEPT BIOQUIM,CASILLA 70086,SANTIAGO 7,CHILE

[2] UNIV CHILE,FAC CIENCIAS,DEPT BIOL,SANTIAGO 7,CHILE

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1991年 / 99卷 / 04期

关键词：

D O I：

10.1016/0305-0491(91)90149-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. The activity of the adenylyl cyclase found in the membranes of Xenopus laevis can be affected by polylysine and other polycations. 2. The activity of the catalytic subunit measured with forskolin is inhibited by polylysine and polyarginine at concentrations above 10-mu-M and by spermine above 3 mM. 3. The adenylyl cyclase activity stimulated by GTP-gamma-S or F- through the stimulatory G protein (G(s)) can be increased by polylysine, polyornithine and spermine but not by polyarginine. 4. Polylysine stimulation of G(s) dependent activity is due to the increase in the apparent affinity for GTP-gamma-S and to a lowering of the requirement for Mg2+ concentration.

引用

页码：827 / 832

页数：6

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