REGULATION OF CHITINASE SYNTHESIS IN TRICHODERMA-HARZIANUM

被引:100
作者
ULHOA, CJ [1 ]
PEBERDY, JF [1 ]
机构
[1] UNIV NOTTINGHAM,DEPT BOT,MICROBIAL BIOCHEM & GENET GRP,NOTTINGHAM NG7 2RD,ENGLAND
来源
JOURNAL OF GENERAL MICROBIOLOGY | 1991年 / 137卷
关键词
D O I
10.1099/00221287-137-9-2163
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The production of chitinase by Trichoderma species is of interest in relation to their use in biocontrol and as a source of mycolytic enzymes. Fourteen isolates of the genus were screened to identify the most effective producer of chitinase. The best strain for chitinase was Trichoderma harzianum 39.1, and this was selected for study of the regulation of enzyme synthesis. Washed mycelium of T. harzianum 39.1 was incubated with a range of carbon sources. Chitinase synthesis was induced on chitin-containing medium, but repressed by glucose and N-acetylglucosamine. Production of the enzyme was optimal at a chitin concentration of 0.5%, at 28-degrees-C, pH 6.0 and was independent of the age of the mycelium. The synthesis of chitinase was blocked by both 8-hydroxyquinoline and cycloheximide, inhibitors of RNA and protein synthesis, respectively. The mode of chitinase synthesis in this fungus is discussed.
引用
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页码:2163 / 2169
页数:7
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