MESSENGER-RNA SYNTHESIS, TRANSPORT, AND STORAGE IN SILKMOTH OVARIAN FOLLICLES

被引：22

作者：

PAGLIA, LM ^{[1
]}

BERRY, SJ ^{[1
]}

KASTERN, WH ^{[1
]}

机构：

[1] WESLEYAN UNIV, DEPT BIOL, MIDDLETOWN, CT 06457 USA

来源：

DEVELOPMENTAL BIOLOGY | 1976年 / 51卷 / 02期

关键词：

D O I：

10.1016/0012-1606(76)90135-4

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Developing oocytes of the silkmoths Actias luna and Antheraea polyphemus contained significant amounts of polyadenylated RNA. This presumed mRNA comprises 0.2-0.3% of the total extractable RNA, and is found exclusively in subribosomal fractions of ooplasm. Pulse-chase and other time-course studies indicate that the nurse cells are the probable source of this as well as other fractions of ooplasmic RNA. Labeled polyadenylated RNA extracted from nurse cells and ooplasm appears in similar, heterodisperse fractions with a major peak at 18 S in sucrose gradients. No evidence of the presence of polysomes or of active protein or RNA synthesis was detected in the oocytes during vitellogenesis.

引用

页码：173 / 181

页数：9

共 28 条

[1] BIOCHEMICAL STUDIES OF NUCLEATE AND ANUCLEATE FRAGMENTS ISOLATED FROM SEA-URCHIN EGGS - A COMPARISON BETWEEN FERTILIZATION AND PARTHENOGENETIC ACTIVATION [J].